Figure 1

Immunohistochemistry of cultured bladder mucosal cells. At primary culture (A, B, C) cells were counter stained to demonstrate morphology (A). A small number of cells were large with a spreading morphology, these stained for CK20 (B). Other cells were more elongated and branching and stained for AO575 (C). Cells were also stained after treatment with MACS beads. Cells that bound to the beads (D, E, F, myofibroblast-like cells) were stained for 5B5 (D), AE1/AE3 (E) and αSMA (F). Cells that eluted from the beads (G, H, I, urothelial cells) were stained for 5B5 (G), A0575 (H) and αSMA (I). (Bar = 100 μm)