Table 1 Selectivity of the individual primary antibodies used to characterise cell populations
From: Immunocytochemical characterisation of cultures of human bladder mucosal cells
Primary Antibody | Dilution used | Antibody selectivity |
|---|---|---|
AE1/AE3 | 1:1000 | Cytokeratins present in epithelial cells |
A0575 | 1:500-1:1000 | Cytokeratins present in epithelial cells |
Cytokeratin 20 (CK20) | 1:200 | Superficial umbrella cells |
α-smooth muscle actin (αSMA) | 1:200 | Smooth muscle, myofibroblasts and myoepithelial cells, not fibroblasts |
5B5 | 1:100 | Fibroblast and myoepithelial cells |