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Table 1 Selectivity of the individual primary antibodies used to characterise cell populations

From: Immunocytochemical characterisation of cultures of human bladder mucosal cells

Primary Antibody Dilution used Antibody selectivity
AE1/AE3 1:1000 Cytokeratins present in epithelial cells
A0575 1:500-1:1000 Cytokeratins present in epithelial cells
Cytokeratin 20 (CK20) 1:200 Superficial umbrella cells
α-smooth muscle actin (αSMA) 1:200 Smooth muscle, myofibroblasts and myoepithelial cells, not fibroblasts
5B5 1:100 Fibroblast and myoepithelial cells