CK2α and uPA are downstream of COX2 in UMUC2 cells. (A), (B) and (D) UMUC2 cells were transfected by control or siRNA of COX2 or CK2α. After 72 h-incubation, mRNA and protein of COX2/COX1, uPA or CK2α were examined by RT-PCR and western blotting. Activity of uPA in culture media was assessed. Cell viability was analyzed by MTS assay. (C) Cell cycle analysis was performed by flow cytometry. Dimethyl sulfoxide was used as control reagent.