COX2 regulates Akt activation through CK2α in UMUC6R cells. (A) UMUC6R cells were obtained from parental line, UMUC6 by long-term incubation under media including doxorubicin. Phosphorylated Akt and Akt were examined by western blotting. (B), (C) and (D) UMUC6R cells were transfected by control siRNA or the indicated siRNA of COX2 or CK2α. After 72 h-incubation, expressions of the indicated gene were examined. Activity of uPA in culture media at 72 h after transfection of control siRNA or siRNA, or at 48 h after treatment with DMSO or specific inhibitor of Akt, LY294002 at 10 μM (for 30 min) was assessed. Cell cycle analysis was performed by flow cytometry. Dimethyl sulfoxide was used as control reagent.