Activation of CK2α-uPA signal independent of COX2 in urothelial carcinoma cells. (A) UMUC3 cells lack COX2 gene were treated by selective COX2 inhibitor, CAY10404(CAY) or DuP-697(DuP) at the indicated concentrations for 48 h, then cell viability was assessed by MTS assay. (B) Cells were treated by DMSO, CAY or DuP at 25 μM for 48 h, and then Cell cycle analysis was performed by flow cytometry. (C) and (D) At 48 h after the same treatments (plus LY294002 treatment) or at 72 h after transfection of control or CK2α siRNA, protein expression (COX2, CK2α, phosphorylated Akt, Akt, p27 or p21) and activity of uPA were examined. Dimethyl sulfoxide was used as control reagent.