Figure 5

COX2 inhibitor suppressed tumor growth through down-regulating CK2α-Akt and uPA signals in vivo. (A) and (B) KU-7-GFP (constitutively over expression of GFP) cells were transplanted into the urinary bladder of nude mice. At 7 days after inoculation, mice were randomized into a control (n = 6), meloxicam (n = 6), treatment groups. COX2 inhibitor, meloxicam was fed (3.0 mg/l). Mice were sacrificed 25 days after treatment; tumor size was quantitatively analyzed by using in vivo and ex vivo imaging. (C) mRNA or protein were extracted from the tumor samples and expression of COX2, phosphorylated Akt, Akt, CK2α, p27 or uPA was examined by western blotting or RT-PCR (left panel). Invasion depth from the top to bottom of implanted tumor foci was measured (right panel).