Immunohistochemical analysis of COX2, CK2α and phosphorylated Akt in human bladder cancer. Expression of COX2, CK2α or phosphorylated Akt was immunohistochemically analyzed. Positive percentages were calculated were classified into four intensities (0, less than 10%; 1, 10-30%; 2, 30-50%; 3, more than 50%). The mean of intensity levels in each stage (pTa, pT1, more than pT2 or CIS (pTis)), or in low or high grade tumor were indicated. Statistical differences of percentages of immunopositive cells between non-invasive/low grade cancer and invasive/high grade phenotype (* p < 0.05, **p < 0.01).