Figure 1
From: Influencing factors on the NMP-22 urine assay: an experimental model
Schematic of experimental model. Low concentration (1×104), medium concentration (1× 105) and high concentrations (1× 106) of UROtsa benign human bladder cells, or a mixture of human bladder cancer lines, RT4, T24, UC-UM-14 and KU-7 were added to 10 ml of pooled urine from three healthy controls. The cellular lysate associated with low protein concentration (3.06 μg), medium concentration (30.6 μg) and high concentrations (306 μg) of UROtsa benign human bladder cells or a mixture of human bladder cancer lines, RT4, T24, UC-UM-14 and KU-7 were added to 10 ml of pooled urine from three healthy controls. In addition, whole blood (1, 5, 20 and 50 μl) and serum (3.06, 30.6 and 306 μg protein) were added to 10 ml of pooled urine from healthy controls.