Figure 1

Schematic representation of S-FISH of cells from cultures or present in biological fluids. Cells are collected by centrifugation at 200 x g for 10 min and the pellet suspended in 100 μl 0.2 N HCl. After washing with PBS, the cells are hybridized in 100 μl hybridization solution containing fluorescent-labeled probes for the SncmtRNA and the ASncmtRNAs. After a stringency washing, the cells are analyzed by fluorescence microscopy.