Figure 2
Hybridization kinetics on T-24 cells. After trypsinization, 105 cells were collected by centrifugation at 200 x g for 10 min and the pellet was subjected to S-FISH as indicated in Figure1. Cells were hybridized simultaneously with probes complementary to the SncmtRNA and ASncmtRNAs for 15, 30, 60 and 120 min. Only the green signal corresponding to the expression of the SncmtRNA was observed, while expression of the ASncmtRNAs was down-regulated (red fluorescence). Cell distribution was revealed with DAPI staining (DAPI). The positive control corresponding to Texas red-labeled 18S rRNA-probe was run in parallel (60x).