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Figure 3 | BMC Urology

Figure 3

From: Determination of the differential expression of mitochondrial long non-coding RNAs as a noninvasive diagnosis of bladder cancer

Figure 3

Stability of the mitochondrial ncRNAs in urine. A, 5 × 104 T-24 cells were loaded in urine obtained from healthy donors and stored at 4°C for 24 h. Cells were then recovered by centrifugation as described and subjected to S-FISH. Only the SncmtRNA (green fluorescence) was detectable, while probes for ASncmtRNAs (red fluorescence) showed absence of signal, as did the corresponding mismatch probes (MM, green or red). The cells were also counterstained with DAPI. A parallel sample was hybridized with a probe specific for the 18S rRNA (18S rRNA). B, DU-145 cells (prostate carcinoma) were subjected to the same S-FISH protocol. Notice that the cells only express the SncmtRNA. No fluorescent signal was obtained with the probes targeted to the ASncmtRNA or with MM probes.

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