Figure 4

Normal proliferating cells express the SncmtRNA and the ASncmtRNAs. A, PHA-stimulated human lymphocytes were hybridized simultaneously with probes complementary to the SncmtRNA and ASncmtRNA. After 15 min of hybridization, strong signals were obtained with both probes in the same cells. No signal was observed with the corresponding mismatch probes (MM, green or red fluorescence). (60x). B, Kidney epithelial cells express both mitochondrial transcripts. The S-FISH revealed that the same cells (as revealed by DAPI staining) expressing the SncmtRNA also express the ASncmtRNAs. The mismatch control (MM) was negative and 18S probe was used as a positive control (60x).