Figure 5From: Inhibition of COX-2 expression by topical diclofenac enhanced radiation sensitivity via enhancement of TRAIL in human prostate adenocarcinoma xenograft model Diclofenac induced the tumor growth delay and enhanced the effect of RT in xenograft mouse model of LNCaP-COX-2. The xenograft mice bearing LNCaP-COX-2 tumor were treated with topical administration of diclofenac gel onto mice skin with cotton swab once a day (day 1–36). The tumors in RT alone group and combination group were exposed to IR with a single dose of 3 Gy on day 3. Tumor volume was measured with calipers and calculated as 0.52LW2. (A) Tumor volume was significantly reduced in combination group. (B) Immunohistochemical staining for COX-2 and Ki-67 was conducted. The expression of COX-2 decreased in diclofenac group and combination group compared with control group, while the expression of COX-2 increased in RT group. The expression of Ki-67 significantly decreased in diclofenac group and combination group. Original magnification, x200.Back to article page