Immunofluorescence labeling for caveolin2 and caveolin3 in rat urinary bladder. Caveolin2 and caveolin3 expression (red) was noted throughout the cell membrane in the smooth muscle layers. Both caveolin 2 and caveolin 3 showed decreased immunoreactivity in the Ovx group but returned to the control value after estrogen treatment, as demonstrated by the Ovx?+?Est group. The horizontal scale bar at the bottom left of each figure indicates the magnification power. The right panels denote the means?±?SD of 5 experiments for each condition determined by relative densitometry. Con?=?control; Ovx?=?ovariectomy; Ovx?+?Est?=?ovariectomy plus 17β-estradiol treatment. *P?<?0.05 vs. control, **P?<?0.05 vs. Ovx.