Immunoblotting of caveolin2 and caveolin3 in the rat urinary bladder. The anti-caveolin 2 and caveolin 3 antibodies recognized 18–20 kDa bands. Anti-GAPDH antibody recognized the 42 kDa band. Caveolin 2 and caveolin 3 protein expressions were significantly decreased in the Ovx group. However, this expression was restored to the level of the control after 17β-estradiol treatment in the Ovx?+?Est group. The lower panels denote the mean?±?standard deviation of 5 experiments for each condition determined by densitometry relative to GAPDH. Con?=?control; Ovx?=?ovariectomy; Ovx?+?Est?=?ovariectomy plus 17β-estradiol treatment; *P?<?0.05 vs. control; **P?<?0.05 vs. Ovx.