Table 2 Antibodies for immunohistochemistry
From: MDM2 SNP309 promoter polymorphism and p53 mutations in urinary bladder carcinoma stage T1
Antibody | Clone | Source | Dilution | Abnormal | Positive |
|---|---|---|---|---|---|
p53 | DO-7 | DAKO | 1:100 | > 10% | Nuclei and cytoplasm |
p21 | SX118 | DAKO | 1:50 | < 10% | Nuclei |
p16 | 6H12 | Novocastra | 1:20 | 0% or > 50% | Nuclei and cytoplasm |
pRb | G3-245 | BD Pharmingen | 1:100 | 0% or > 50% | Nuclei |