Figure 1

Schematic representation of the in vitro model. The organ bath was continuously perfused with Krebs’ solution. Chemical stimulation was performed by perfusion the bath with 80 mM KCl or 1 μM CCh and flushing the bath with Krebs’ solution in between. During BoNT-A incubation, the bath was continuously perfused by the same concentration of BoNT-A (0.03, 0.2, 0.3 nM).