Table 1 Sequence of primers used in qRT-PCR
From: microRNA-99a acts as a tumor suppressor and is down-regulated in bladder cancer
Primer | Sequence(5′ → 3′) |
|---|---|
miR-99a-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAGA |
miR-99a-forward | GCTGGAGAACCCGTAGATCCGAT |
miR-99a-reverse | GTGCAGGGTCCGAGGT |
miR-99a-probe | FAM-ATACGACCACAAGATCGG-MGB |
U6-RT | AAAATATGGAACGCTTCACGAATTTG |
U6-forward | CTCGCTTCGGCAGCACATATACT |
U6-reverse | ACGCTTCACGAATTTGCGTGTC |
U6-probe | FAM-CCATGCTAATCTTCTCTGTA-MGB |