Table 1 Cell population fractions (%). Cumulative frequency distributions were examined to extract cell cycle and ploidy sub-populations. Diploid fraction corresponds to a DNA normal 2C non-cycling DNA content. Diploid S fractions have DNA content between diploid and tetraploid values. The tetraploid sub-population contains both diploid G2/M cells and tetraploid G1 cells. The cells with DNA content between tetraploid and octaploid are S-phase tetraploid cells, Octaploid DNA content cells are the G2/M cells of the tetraploid population.
From: Interstitial cystitis antiproliferative factor (APF) as a cell-cycle modulator
Treatment | Dose (μl) | Diploid | Diploid S | Tetraploid | Tetraploid S | Octaploid |
|---|---|---|---|---|---|---|
APF | 10 | 57.27 | 8.72 | 28.76 | 1.54 | 3.72 |
APF | 10 | 58.87 | 3.88 | 32.26 | 1.18 | 3.81 |
APF | 20 | 58.82 | 4.80 | 30.78 | 1.18 | 4.43 |
APF | 20 | 58.17 | 8.44 | 30.02 | 1.07 | 2.31 |
APF | 50 | 40.11 | 2.23 | 51.40 | 0.56 | 5.71 |
APF | 50 | 34.89 | 4.68 | 54.24 | 1.08 | 5.11 |
Mock | 10 | 87.72 | 1.64 | 9.60 | 0.50 | 0.55 |
Mock | 10 | 90.14 | 1.83 | 7.13 | 0.25 | 0.64 |
Mock | 20 | 90.60 | 2.05 | 6.77 | 0.34 | 0.24 |
Mock | 20 | 91.14 | 1.47 | 6.80 | 0.15 | 0.44 |
Mock | 50 | 73.08 | 5.98 | 20.75 | 0.00 | 0.19 |
Mock | 50 | 72.45 | 5.85 | 20.22 | 0.40 | 1.09 |