Figure 1

HPV DNA detection in prostate carcinomas. PCR reactions using primers MY09 and MY11 were performed. After gel electrophoresis and transfer, the filter was hybridized with consensus probes. Positive (SiHa) and negative (HaCaT, water) controls were included in the experiment, but are not shown in the figure. The numbers above the lanes indicate the designation of the carcinoma samples. The bar on the left side indicates the position of the MY09/11 PCR product of approximately 450 bp length. Two hybridization signals are visible in the HPV-positive samples. The upper band corresponds to the MY09/11 PCR product. The additional lower band which was also seen in other experiments using these batches of the MY09/11 primers, appeared exclusively as a companion of the HPV-specific 450 bp product. We did not try to clarify the nature of this extra-band.