Table 2 PCR primers used for the analysis of p53 polymorphism at codon 72.
Allele | Primer | Primer sequence | PCR product size |
|---|---|---|---|
p53-Pro72 | p53 Pro + | 5'GCCAGAGGCTGCTCCCCC3' | 178 bp |
p53- | 5'CGTGCAAGTCACAGACTT3' | ||
p53-Arg72 | p53+ | 5'TCCCCCTTGCCGTCCCAA3' | 136 bp |
p53 Arg- | 5'CTGGTGCAGGGGCCACGC3' |