Figure 7

Cell fractionation and western blot of LNCaP cells treated with Casodex. Cell fractionation of cytosolic (Cyto) and mitochondrial (Mito) fractions and western blot was performed as described in Materials and Methods. LNCaP cells either treated with 1 μM Casodex for 5 weeks or control were fractionated and subjected to western blot and show that LNCaP-UR cells overexpress BNIP3 which is predominately localized in the mitochondrial fraction. Lane 1, LNCaP-R control cytosolic fraction. Lane 2, LNCaP-R control mitochondrial fraction. Lane 3, LNCaP-R treated with 1 μM Casodex for 5 weeks, cytosolic fraction. Lane 4, LNCaP-R treated with 1 μM Casodex for 5 weeks, mitochondrial fraction. Lane 5, LNCaP-UR control cytosolic fraction. Lane 6, LNCaP-UR control mitochondrial fraction. Lane 7, LNCaP-UR treated with 1 μM Casodex for 5 weeks, cytosolic fraction. Lane 8, LNCaP-UR treated with 1 μM Casodex for 5 weeks, mitochondrial fraction. TOM20, a mitochondrial integral membrane protein, (bottom panel) was included as a control.