Figure 1

The effect of R1881 on viable cell number, cell cycle and glycogen content of PC3-AR-V and PC3-AR-E7 cells. Two independently isolated control (PC3-AR-V1 and PC3-AR-V2) or HPV-E7 expressing clones (PC3-AR-E72 and PC3-AR-E73) were tested. R1881 was added to exponentially growing cells to a final concentration of 4 nM and cells were further incubated for the indicated times. PC3 cells, which lack AR were used as a negative control. Cell samples were removed A) to determine the glycogen content and B) to determine the viable cell count using the trypan blue assay.