Figure 3

Determination of glycogen synthase activities in PC3-AR-E7 cells. R1881 was added to cells to a final concentration of 4 nM. Samples were removed at the indicated times. Activities are in nMol/min/mg protein. A) GS activity was determined from cells treated with R1881 for 72 hours and from untreated cells. GS activities were determined in the absence (-G-6-P) and presence (+G-6-P) of G-6-P. B) GS activities in the presence of G-6-P was determined from cells treated with R1881 for the times indicated. C) The G-6-P content was determined in untreated and 24 hours R1881-treated PC3-AR-E7 cells.