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Figure 3 | BMC Urology

Figure 3

From: Substance P induces localization of MIF/α1-inhibitor-3 complexes to umbrella cells via paracellular transit through the urothelium in the rat bladder

Figure 3

Colocalization of MIF/A1-I3 in umbrella cells after SP-treatment. Dual-immunofluorescence for A1-I3 and MIF in bladder sections from saline (A, B) and SP-treated rats (C–H). Bladders from saline-treated rats showed no A1-I3 in the urothelium (A; edge of unstained urothelium marked by long arrows) while the lamina propria (lp) showed significant A1-I3 immunostaining. On the other hand, MIF is present in the urothelium of saline-treated rats (B), mainly in basal and intermediate cells. Representative sections from three (3) different animals show that after substance P treatment, A1-I3 immunostaining is seen in umbrella cells (C, E, G; arrows) that also co-localized MIF (D, F, H; arrows). In addition, increased staining in the lamina propria (lp) can be observed (D, F, H). Asterisk (H) shows area of superficial urothelium with MIF immunostaining but lacking A1-I3 immunostaining (G). Calibration bar = 100 um.

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BMC Urology

ISSN: 1471-2490

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