Figure 9

Intravesical SP stimulates NKx-2.5 and NF-kappaB activity. Anesthetized mice were instilled with 200 μl of saline or SP (10 μM) and bladders were removed 2, 6, and 24 hours after instillation. In one additional group (zero hours) the urinary bladders were removed without instillation. Urinary bladders were placed in cold phosphate buffered saline (0°C) containing protease inhibitors, and the mucosa was dissected away from the muscle. Nuclear proteins were extracted and used for electrophoretic mobility shift assay for NKx-2.5 (A) and NF-kappaB (C). Amount of shifted Nkx-2.5 (B) and NF-kappaB (D) probes were quantified as described in materials and methods.