AR expression and DHT response in bladder. Nuclear AR expression (brown-stained cells) in (A, B) human bladder cancer and (C) benign urothelium at 400× original magnification. Dako antibody clone AR441 was used for labelling. (D) MTT assay of human prostate cell lines (LNCaP and PC3), human bladder cancer cell lines (J82 and TCCSUP), and male mouse urothelial explant from UPII-SV40T animal (MMUST). Cells were treated with ethanol or DHT in ethanol vehicle for 72 hours prior to MTT assay. Experiments done in triplicate. Values are relative means ± SE. Western blot analysis using Santa Cruz 815 antibody was performed to determine AR expression.