Immunohistochemical staining of mouse bladder. Male UPII-SV40T animals were sham-operated (intact) or castrated at 24 weeks of age, and then animals were sacrificed at 32 weeks and bladders harvested, formalin-fixed, and paraffin embedded. The upper panel shows similar levels of SV40T staining in the urothelium of both intact and castrated animals. TSP1 expression is indicated by arrows in the center panels. AR is also expressed in the urothelium of both intact and castrated animals (anti-AR, Santa Cruz 815). Magnification: 200× original.