TSP1 protein expression in mouse bladder. Whole bladder lysates of sham-operated (intact) wild-type and sham-operated (intact) or castrated UPII-SV40T male mice were assayed and quantified for TSP1 expression by western blot analysis. (A) Relative bladder TSP1 protein expression in wild-type vs. UPII-SV40T mice. Values are relative means ± SE. For each group, n = 3. p = 0.0326*. (B) Relative bladder TSP1 protein expression in UPII-SV40T mice sacrificed 2, 3, 7, and 17 days after castration. Intact relative expression = 1. Values are relative means ± SE. For each group, n = 3. p = 0.0154** vs. intact. (C) Western blot of intact (n = 3) vs. 17-day castrate (n = 3) whole bladders from UPII-SV40T mice. TSP1 immunoblots were quantified. GAPDH control blot verified equal loading. P values determined by paired, two-tailed, student's t-test.