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Figure 8 | BMC Urology

Figure 8

From: Protein kinase C modulates frequency of micturition and non-voiding contractions in the urinary bladder via neuronal and myogenic mechanisms

Figure 8

Both low and high PKC stimulation reduces sensitivity of BSM strips to stimulation of muscarinic receptors by carbachol. A, Raw tracers of contractile responses to increasing concentrations of carbachol in the control (top panel) isolated BSM strip, and strips treated with low (middle panel, 50 nM) and high (bottom panel, 1 μM) concentration of PDBu. B, Both low and high PDBu caused a significant rightward shift (reduced sensitivity) in the carbachol concentration response curve. The EC50 values were 0.56 ± 0.049, 4.2 ± 0.35, and 4.7 ± 0.47 nM for control (black circles), PDBu (50 nM, open circles, N = 4, n = 6, p ≤ 0.05 to control), and PDBu (1 micromolar (1uM), grey circles p ≤ 0.05 to control)), respectively. C, Despite the reduced sensitivity of BSM strips to carbachol stimulation after PDBu treatment, there was no significant difference in the PF of contractions between the groups. The PF values were 2.2 ± 0.25, 2.1 ± 0.24, and 2.0 ± 0.18 g for control, PDBu (50 nM), and PDBu (100 nM) groups, respectively.

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