Fig. 2

Actin and vimentin or P2X receptor immunostaining in human urinary bladder smooth muscle layer. a. Costaining (yellow) of the smooth muscle layer with actin (red) and vimentin (green), with scatterplot of red and green pixel intensities. Section is from a patient with prostate cancer. b-c. Smooth muscle layer double labelled with P2X (green) and actin (red) with scatterplot of red and green pixel intensities. Sections are from a patient with bladder cancer. d. Pearson’s correlation coefficient for actin (red channel) and P2X/vimentin (green channel) expressed as mean ± SEM (n = 9). *p < 0.017 and **p < 0.0017 for Bonferroni adjusted levels of significance. Scale bar = 100 μm