Table 2 Forward and reverse primers of ROCK1, ROCK2, MLCK and GAPDH (from Molbiol). ACTB and PGK1 were detected with Qiagen Primer Assays
Gene name | Forward primer | Reverse primer | PCR product |
|---|---|---|---|
ROCK1 | AAAATTGTGTGAGGAGGACATGG | TTCATCCCAACATTCTTGGATCT | 279 bp |
ROCK2 | GCAATGCGGTAAAAAGCGA | GGGAATCATGGTGTGACCAA | 217 bp |
MLCK | GTCTTATGTTATCTTCCATTCTA | TATAATAAACTGTGGCAATACTG | 156 bp |
GAPDH | AGAAGGCTGGGGCTCATTTG | AGGGGCCATCCACAGTCTTC | 285 bp |