Fig. 1
From: Regulation of urinary bladder function by protein kinase C in physiology and pathophysiology
Effects of carbachol (CCh) on the contractile force and spontaneous contractions in DSM in vitro. Isolated muscle strips from rabbit bladders were mounted in organ baths with Tyrode’s buffer (equilibrated with 95%O2/5%CO2) and allowed to develop spontaneous contractions. The muscle strip shown in a was treated with 20 μM of carbachol while the muscle strip in b was first pre-incubated with Bim-1 (28 nM), a PKC inhibitor, for 45 min prior to adding carbachol. Treatment with carbachol increased peak muscle force and reduced the amplitude of spontaneous contraction (SCA) by 96 ± 14 %, (n = 4, p < 0.05 in a), however, carbachol had no effect on spontaneous contraction amplitude (SCA) after PKC inhibition with Bim-1 (b). c Normalized amplitude of the peak force of the contractile response to CCh. Maximal amplitude was taken as 100 %. Light bars show the effects of carbachol on peak force and SCA without Bim-1. Dark bars show the effect of carbachol on peak force and SCA with Bim-1 application