Regulation of urinary bladder function by protein kinase C in physiology and pathophysiology

Hypolite, Joseph A.; Malykhina, Anna P.

doi:10.1186/s12894-015-0106-6

BMC Urology

Table 1 Effects of PKC signaling on bladder function

From: Regulation of urinary bladder function by protein kinase C in physiology and pathophysiology

PKC Drugs	Tissue	Methods	Physiological condition	Species	Effect on bladder function	Reference
PBBu	Muscle strips	In vitro	Normal	Rabbit, Rat	Inhibition of spontaneous contraction	[8]
(PKC activator, 1nM–50nM)	Muscle strips	In vitro	Normal	Rabbit, Rat	Inhibition of spontaneous contraction	[22]
PDBu	Muscle strips	In vitro	Normal	Rabbit	Increased contractility	[8, 25, 28]
(100nM-1 μM)	Muscle strips	In vitro	Normal	Rabbit	Increased contractility	[8, 25, 28]
PDBu	Muscle strips	In vitro	Normal	Rat	Increased nerve sensitivity, force maintenance	[8, 22, 31]
(100nM-1 μM)	Muscle strips	In vitro	Normal	Rat	Increased nerve sensitivity, force maintenance	[8, 22, 31]
PDBu	Bladder	In vivo (cystometry)	Normal	Rat	Bladder emptying, and frequency of urination	[22]
(1 μM)	Bladder	In vivo (cystometry)	Normal	Rat	Bladder emptying, and frequency of urination	[22]
PDBu	Muscle strip	In vitro	Normal	Rabbit	Activation of ROCK, Increased DSM tone	[28]
PDBu	Muscle strips	In vitro, EFS	Normal	Rat	Increased peak force and area; increased release of acetylcholine.	[30]
PDBu	Bladder myocytes	In vitro	Normal	Mice	Increased Cav1.2 current	[33]
PDBu	α-toxin muscle strips	In vitro	Normal	Guinea pig	Increased Ca²⁺ sensitivity	[80]
PDBu	Muscle strips	In vitro	Obese	Mice	Increased contractility	[26]
(0.001–3 μM)	Muscle strips	In vitro	PBOO	Mice	Increased contractility	[60]
PDBu	Muscle strips	In vitro	PBOO	Rabbit	Decreased contraction and PKC activity	[25, 27]
PMA	DSM cells & Muscle strips	In vitro,	Normal	Guinea pig	Inhibition of K_ATP and BK currents; increased DSM contraction	[22, 31, 47]
(PKC activator)	DSM cells & Muscle strips	Patch-clamp recordings	Normal	Guinea pig		[22, 31, 47]
Bim-1	Muscle strips	In vitro	Normal	Rabbit	Increased spontaneous contractions; decreased force maintenance; decreased peak force; decreased void; increased non-voiding contractions	[8, 22, 24]
(PKC inhibitor)	Bladder	In vivo	Normal	Rat		[8, 22, 24]
Ro318220	Muscle strips	In vitro	Normal	Rat	Inhibition of force maintenance;	[22]
(PKC inhibitor)	Muscle strips	In vitro	Normal	Rat	Inhibition of force maintenance;
(PKC inhibitor)	Bladder	In vivo	Normal	Rat	Increased non-voiding contractions; decreased void volume
GF109203X	Muscle strips	In vitro	Normal	Guinea pig; rat	Inhibition of Ca²⁺ sensitization	[17]
(PKC inhibitor)	Muscle strips	In vitro	Normal	Guinea pig; rat	Inhibition of Ca²⁺ sensitization	[17]
Carbachol	DSM cells	In vitro, Patch-clamp recordings	Unknown	Human	Inhibition of BK channels	[76]
Carbachol	DSM	In vitro	Unknown	Human	Increased Ca++ sensitivity of contractile filaments	[68]

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ISSN: 1471-2490

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