Fig. 5

The expression of Cyclin D1, CDK4 and CDK6 were down-regulated by Glucosamine. When 786-O (a, b) and Caki-1 (c, d) cells were treated with Glucosamine for 24 h, the expression of Cyclin D1, CDK4 and CDK6 were down-regulated. Cells were lysed with RIPA lysis buffer, and the lysates were then analyzed by Western blot. a, c Representative gels from 3 experiments are shown. b, d Densitometric analysis was performed for each protein band relative to β-actin in the same sample using Quantity one software. *P < 0.05 compared with the control group (0 mM)