Table 1 The primer sequences of PDE isoforms
From: Expression and function of phosphodiesterases (PDEs) in the rat urinary bladder
Name | Sequences |
|---|---|
PDE1A (Forward, F) | 5′CCACTTTGTGATCGGAAGTC3′ |
PDE1A (Reverse, R) | 5′TTCTGCTGAATGATGTCCACC3′ |
PDE1B (F) | 5′CAGGGTGACAAGGAGGCAGAG3′ |
PDE1B (R) | 5′GACATCTGGTTGGTGATGCC3′ |
PDE1C (F) | 5′TCTCAAAGGATGACTGGAGG3′ |
PDE1C (R) | 5′GCTTCTCTGTCACCCTGTC3′ |
PDE2A (F) | 5′CCTCCTGTGACCTCTCTGACC3′ |
PDE2A (R) | 5′TGAACTTGTGGGACACCTTGG3′ |
PDE3A (F) | 5′TCACAGGGCCTTAACTTACAC3′ |
PDE3A (R) | 5′GGAGCAAGAATTGGTTTGTCC3′ |
PDE3B (F) | 5′CCTCAGGCAGTTTTATACAATG3′ |
PDE3B (R) | 5′TGCTTCTTCATCTCCCTGCTC3′ |
PDE4A (F) | 5′GTGGAGAAGTCTCAGGTGGG3′ |
PDE4A (R) | 5′TGGAACTTGTCAGGCAGGG3′ |
PDE4B (F) | 5′TAGAAGATAACAGGAACTGG3′ |
PDE4B (R) | 5′GCAATGTCTATGTCAGTCTC3′ |
PDE4C (F) | 5′ACGTGGCGTACCACAACAGC3′ |
PDE4C (R) | 5′TACCGCGAGGTGATGGTTCTC3′ |
PDE4D (F) | 5′GGATAATGGAGGAGTTCTTCC3′ |
PDE4D (R) | 5′CGATTGTCCTCCAAAGTGTCC3′ |
PDE5A (F) | 5′CCCTGGCCTATTCAACAACGG3′ |
PDE5A (R) | 5′GTGGGTCAGGGCCTCATACAG3′ |
PDE7A (F) | 5′TGGACAAGCCAAGTGTATGCTG3′ |
PDE7A (R) | 5′TTTAAGTAACAGTGCATGGCC3′ |
PDE7B (F) | 5′AAAGCCCAGTGGAAGAGC3′ |
PDE7B (R) | 5′CGAAGGGAGGTGGTAAATG3′ |
PDE8A (F) | 5′CAACAAGCCTCTGAAAGC3′ |
PDE8A (R) | 5′TCGGTCTGGGAGAAATAC3′ |
PDE8B (F) | 5′ACCACAACTCCACCCATG3′ |
PDE8B (R) | 5′AGAGGCTTGTTGATGCTG3′ |
PDE9A (F) | 5′TGGGTGGACTGTTTACTGG3′ |
PDE9A (R) | 5′CGGTCTTCATTGTCTTTCG3′ |
PDE10A (F) | 5′TGCTTGGTGGCGTTTGTTAG3′ |
PDE10A (R) | 5′TTCTCTGATGCCTGGGATGTAC3′ |
PDE11A (F) | 5′TTTAGCGGTGATTGTGGG3′ |
PDE11A (R) | 5′TCTCGAAGTACAGCGTGAGG3′ |
GAPDH (F) | 5′CAAGTTCAACGGCACAGTCAAG3′ |
GAPDH (R) | 5′GCACCAGTGGATGCAGGGAT3′ |