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Fig. 3 | BMC Urology

Fig. 3

From: Site-dependent differences in the composite fibers of male pelvic plexus branches: an immunohistochemical analysis of donated elderly cadavers

Fig. 3

Immunohistochemistry of nerves in an area between the rectum and seminal vesicle (middle and superior group of the pelvic plexus branches). Topographical nerve anatomy is shown in Fig. 1b. Panels a, d, f, and h display nNOS immunostaining, while panels c, e, g, and i exhibit TH immunostaining. Only panel b displays VIP immunostaining: the other nerves in this figure did not contain VIP-positive fibers (not shown). Panels ac (or f and g; h and i) are adjacent sections, whereas panels d and e are proximal sections. Arrows, arrowheads, and stars in panels ac indicate ganglion cells corresponding to each panel: two ganglion cells (stars) appear to be negative for all three markers. All VIP-positive cells (panel b) appear to express nNOS (panel a). All TH-positive cells (panel c) do not express either nNOS or VIP. In all of these nerves, nNOS- and TH-positive fibers appear to be intermingled and do not exhibit a clear localization. All panels were prepared at the same magnification (scale bar in panel a, 0.1 mm)

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