Fig. 3From: Genomic case report of a low grade bladder tumor metastasis to lunga Cells from the lung metastasis were treated with Everolimus at 10 and 50Â nM concentrations for 24Â h. The cells were lysed, and Western blots were carried out for proteins involved in mammalian target of rapamycin (mTOR) signaling. b 103 cells were plated in triplicates in 96-well plate followed by treatment with increasing concentrations of everolimus. Cytotoxicity assay was carried out after 72-h drug treatment as described in Materials and MethodsBack to article page