Fig. 1

Genotyping of WT and TREK-1 KO mice. a Representative agarose gel showing the amplicons of wild-type (C57BL/6 J, WT, N = 1) and TREK-1 KO (KO, N = 3) alleles. The first column represents the molecular weight marker (MW) with the following three lines corresponding to the amplicon products generated by KO mRNA. The fourth line illustrates the amplicon produced by WT mRNA for comparison. The size of the bands corresponding to the TREK-1 KO (KO) and WT alleles are around ~ 200 and ~ 450 bp, respectively, as previously reported [45]. b Western blotting of total bladder protein from WT and TREK-1 KO animals showing a prominent band at ~ 50–52 kDa in WT mice but not in TREK-1 KO group (KO). Histone H3 was used as loading control