Fig. 3

Increased basal tone and amplitude of spontaneous contractions in the detrusor of TREK-1 KO mice. a The basal tone of BSM strips isolated from WT (N = 17, n = 34) and TREK-1 KO (N = 17, n = 34, KO) mice was measured at L_0. Incubation with arachidonic acid (AA) significantly reduced the basal tone in WT (WT + AA, N = 4, n = 8, p ≤ 0.05) but not in TREK-1 KO group. b Application of additional stretch (30% to L₀) caused an increase in basal tone of both WT and KO strips. No significant changes in response to TREK-1 inhibitor, L-methionine (LM), were recorded in both groups. c The amplitude of spontaneous contractions at L₀ was significantly elevated in TREK-1 KO group in comparison to WT group. The insert shows zoomed-in raw traces from both experimental groups. d Stretch protocol increased the amplitude of spontaneous contractions in both WT and TREK-1 KO BSM strips. * - p ≤ 0.05 to respective WT group, # - p ≤ 0.05 within the group