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Fig. 6 | BMC Urology

Fig. 6

From: Altered detrusor contractility and voiding patterns in mice lacking the mechanosensitive TREK-1 channel

Fig. 6

Activation of PKC pathway in the urinary bladder is associated with higher response to stimulation in TREK-1 KO mice. a Effects of PKC activator, PDBu (1 μM) on contractile function of WT (N = 8, n = 8) and TREK-1 KO (N = 8, n = 9, left panel) muscle strips. Right panel indicates that PDBu induced a significant increase in PF in TREK-1 KO group when compared to WT bladders. b RT-PCR results show gene expression of 7 major PKC isoforms expressed in the urinary bladders from both WT and TREK-1 KO mice. Bands from 1 to 7 represent the following PKC isoforms: 1 - PKC-α (alpha), 2 - PKC-β (beta), 3 - PKC-γ (gamma), 4 - PKC- δ (delta), 5 - PKC-ε (epsilon), 6 – PKC- μ (mu), 7- PKC-τ (tau). Line 8 - GAPDH. M- marker, * - p ≤ 0.05 to WT group

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