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Table 2 TaqMan gene expression assays used for real-time PCR expression analysis

From: Expression of components of the urothelial cholinergic system in bladder and cultivated primary urothelial cells of the pig

Assay

Order No.

VAChT-Pig

Custom Gene ex assay, AIWR3FN, referred to the cDNA sequence with GenBank Accession No. XM_013983446

ChAT-Pig

Taqman Gene ex assay MTO, sm, Ss03391504_m1

CarAT-Pig

Taqman Gene ex assay MTO, sm, Ss03389781_m1

ABCB1-Pig

Taqman Gene ex assay MTO, sm, Ss03373435_m1

OCT1-Pig

Taqman Gene ex assay MTO, sm, Ss03391173_m1

OCT2-Pig

Taqman Gene ex assay MTO, sm, Ss03390935

OCT3-Pig

Custom Gene ex assay, JD89KCA, referred to the cDNA sequence with GenBank Accession No. XM_003121106

OATP1A2-Pig

Taqman Gene ex assay MTO, sm, Ss03375623_u1

CHRM1-Pig

Taqman Gene ex assay MTO, sm, Ss03393581_u1

CHRM2-Pig

Taqman Gene ex assay MTO, sm, Ss03383697_u1

CHRM3-Pig

Taqman Gene ex assay MTO, sm, Ss03387661_u1

CHRM4-Pig

Custom plus Tqmn RNA Assays, AJPADK0, referred to the cDNA sequence with GenBank Accession No. XM_003122828

CHRM5-Pig

Custom plus Tqmn RNA Assays, AJN1FES, referred to the cDNA sequence with GenBank Accession No. XM_021099908

GAPDH-Pig

FG, Off the shelf GX Set, Ss03374854_g1