Table 1 Sequence of 10 sets of primer employed to identify Y-chromosome microdeletions
STS/gene | Primer sequence | Product size (bp) | Region |
|---|---|---|---|
SRY-F | 5′ - GAA TAT TCC CGC TCT CCG GA - 3′ | 495 | NRY |
SRY-R | 5′ - GCT GGT GCT CCA TTC TTG AG - 3’ |  |  |
ZFY-F | 5′ - ACC RCT GTA CTG ACT GTG ATT ACA C - 3’ | 472 | NRY |
ZFY-R | 5′ - GCA CYT CTT TGG TAT CYG AGA AAG T - 3’ |  |  |
sY86-F | 5′ - GTG ACA CAC AGA CTA TGC TTC - 3’ | 320 | AZFa |
sY86-R | 5′ - ACA CAC AGA GGG ACA ACC CT - 3’ |  |  |
sY127-F | 5′ - GGC TCA CAA ACG AAA AGA AA - 3’ | 274 | AZFb |
sY127-R | 5′ - CTG CAG GCA GTA ATA AGG GA - 3’ |  |  |
sY254-F | 5′ - GGG TGT TAC CAG AAG GCA AA - 3’ | 400 | AZFc |
sY254-R | 5′ - GAA CCG TAT CTA CCA AAG CAG C - 3’ |  |  |
sY1161-F | 5′ - CGA CAC TTT TGG GAA GTT TCA - 3’ | 377 | AZFc |
sY1161-R | 5′ - TTG TGT CCA GTG GTG GCT TA- 3’ |  |  |
sY1191-F | 5′ – CCA GAC GTT CTA CCC TTT CG - 3’ | 385 | AZFc |
sY1191-R | 5′ – GAG CCG AGA TCC AGT TAC CA - 3’ |  |  |
sY1201-F | 5′ – CCG ACT TCC ACA ATG GCT - 3’ | 677 | AZFc |
sY1201-R | 5′ – GGG AGA AAA GTT CTG CAA CG - 3’ |  |  |
sY1206-F | 5′ – ATT GAT CTC CTT GGT TCC CC - 3’ | 394 | AZFc |
sY1206-R | 5′ – GAC ATG TGT GGC CAA TTT GA - 3 |  |  |
sY1291-F | 5′ – TAA AAG GCA GAA CTG CCA GG - 3’ | 527 | AZFc |
sY1291-R | 5′ – GGG AGA AAA GTT CTG CAA CG - 3’ |  |  |