Fig. 2

miR-185 inhibited ALK4 expression by binding to the 3′-UTR of ALK4. a Schematic of the candidate miRNAs that target ALK4 predicted by StarBase. b Dual-luciferase assay was performed to study the interaction between miR-185 and ALK4. The expression levels of miR-185 c and ALK4 d were quantified by qRT-PCR in DU145 cells and LNCaP cells transfected with miR-185 mimics or miR-185 inhibitor. e Protein levels of ALK4 were assessed by Western blotting in DU145 cells or LNCaP cells with the indicated treatments. The data represent one of three independent experiments. Error bars denote the mean ± SD. *P < 0.05, **P < 0.01