Fig. 3
From: miR-185 inhibits prostate cancer angiogenesis induced by the nodal/ALK4 pathway
MiR-185 targeted ALK4 to regulate angiogenesis induced by prostate cancer cells. a Protein levels of VEGF were assessed by Western blot in DU145 cells and LNCaP cells transfected with miR-185 mimics or pcDNA3.1-ALK4. b Representative photomicrographs of the colony formation assay and quantification of colony numbers in DU145 cells and LNCaP cells transfected with miR-185 mimics or pcDNA3.1-ALK4. c Cell viability of co-cultured HUVECs was evaluated by MTT assay and was determined as the percentage of the value relative to that of the control group. d The migration ability of co-cultured HUVECs was detected by transwell assay. e Tube forming ability of HUVECs was observed after the indicated treatment. The data represent one of three independent experiments. Error bars denote the mean ± SD. *P < 0.05, **P < 0.01