Fig. 4

MTA1 regulated the expression of metastasis-related factors via the NFκB pathway. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. a Protein levels of MTA1; cells were collected for WB with MTA1 and GAPDH antibodies. The bands of over-expressed MTA1 were from Additional file 1: Fig. 2A and down-expressed MTA1 were from Additional file 1: Fig. 2B with arrow. b Cells were lysed and used in qRT-PCR assays to measure the E-cadherin mRNA expression. Statistical analysis was performed using Student’s t test. b, c A498 cells were transfected with pcDNA3.1-Flag and Flag-MTA1. After 36 h, the Flag-MTA1 group was treated with 10 nM PDTC for 12 h. Then cells were harvested for qRT-PCR evaluation to measure the mRNA expression of MMP2 (b) and MMP9 (c). Statistical analysis was performed using ANOVA. d A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 48 h, cells were subjected to western blotting analysis using antibodies targeting p-p65, p65 and ACTB. The bands of p-p65, p65 and ACTB were from Additional file 1: Fig. 3. e, f Image J was used to calculate the gray scanned bands of p-p65 (b) and p65 (C). *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001; NS  not significant