Fig. 3

miR-381 targets BMI1 to inactivate the Rho/Rock pathway. a target relationship between miR-381 and BMI1 predicted on StarBase (http://starbase.sysu.edu.cn/) and validated through a dual luciferase reporter assay (*p < 0.05, two-way ANOVA); b BMI1 expression in BCa tissues determined by RT-qPCR (*p < 0.05, two-way test); c BMI expression in BCa cell lines (T24 and RT4) and in normal bladder epithelial cells determined by RT-qPCR (*p < 0.05, one-way ANOVA); d correlation analysis between miR-381 and BMI1 expression in BCa tumor tissues; e BMI1 expression in T24 and RT4 cells after miR-381 mimic transfection measured by RT-qPCR (*p < 0.05, two-way ANOVA); f BMI1 expression in BCa tumor tissues after BMI1-OE vector transfection detected by RT-qPCR (*p < 0.05, two-way ANOVA). g protein levels of ROCK2, RhoA and RhoA-GFP (phosphorylated RhoA) in each group of cells measured by western blot analysis (*p < 0.05, two-way ANOVA); Repetition = 3