Fig. 5

BMI1 partially blocks the role of miR-381. a cells pre-transfected with miR-381 mimic were further transfected with BMI1-OE vectors, and it was found that BMI1-OE#3 presented the best overexpressing efficiency (*p < 0.05, two-way ANOVA); b optical density at 450 nm of each group of cells according the CCK-8 method (*p < 0.05, two-way ANOVA); c migration distance at the 24th hour measured by the scratch test (*p < 0.05, two-way ANOVA); d number of invaded cells at the 24th h determined by the transwell assay (*p < 0.05, two-way ANOVA); e number of PI-Annexin V-double positive cells detected by flow cytometry (*p < 0.05, two-way ANOVA); f tumor growth rate change and weight on the 28th day in nude mice (n = 8) (*p < 0.05, two-way ANOVA); Repetition = 3