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Table 3 CTC counts of aneuploidy of chromosome 8 and/or 7 from blood samples

From: CTCs detection from intraoperative salvaged blood in RCC–IVC thrombus patients by negative enrichment and iFISH identification: a preliminary study

Case Sample CTC counts
Triploid of chromosome 8 Tetraploid of chromosome 8 Polydiploid (> 4) of chromosome 8 Triploid of chromosome 7 Tetraploid of chromosome 7 Polydiploid (> 4) of chromosome 7 Polydiploidy chromosome of both 7 and 8 CTM Sum Total number
1 S1 3         3  
S2           
S3 1         1  
S4           
           4
2 S1 1    2    1a   4  
  S2           
  S3     1      1  
  S4           
            5
3 S1 6 1   3      10  
S2 1    1     1 3  
S3           
S4           
           13
4 S1 4    3      7  
S2 3    1    1a   5  
S3           
S4           
           12
5 S1 1    1    1a   3  
S2     1      1  
S3           
S4           
           5
  1. Tumor cells were recognized and counted using immunostaining and fluorescence in situ hybridization technology. Only cells with CD45−/DAPI+/CEP8+ (> 2), CD45−/DAPI+/CEP8+ (> 2), and CD45−/DAPI+/CEP8+ and CEP7+ (hyperdiploidy) were identified as CTCs
  2. Number 0 were not shown in the table
  3. S1, peripheral venous blood sample from internal jugular vein before skin incision, indicative of the circulating tumor cells; S2, blood sampled in the vena cava around thrombus during surgery, indicating direct cells shedding in surgical manipulation; S3, blood after IOCS washing and before LDF filtering, which represents the tumor cell cleaning efficacy with only IOCS; S4, blood sampled after LDF filtration, meaning the actual final filtering result after IOCS–LDF
  4. CTM circulating tumor microemboli
  5. aTriploid of both chromosome 8 and 7