Fig. 3

DLX1 is a target gene of miR-4429 in PCa cells. a Putative binding site between miR-4429 and the DLX1 3′-UTR. b–c PCa expression (b) and its relevance to the overall survival of patients (c) predicted according to the data on the GEPIA database (d). Binding relationship between miR-4429 and DLX1 3′-UTR evaluated via a dual luciferase reporter gene analysis. DLX1 3′-UTR luciferase vector and miR-4429 mimic or NC mimic were co-transfected into DU145 and PC-3 cells and incubated for 48 h before measuring luciferase activity (one-way ANOVA, P < 0.0001). e miR-4429 mimic/inhibitor or NC mimic/inhibitor transfected into DU145 and PC-3 cells for 48 h, and DLX1 mRNA expression was measured by RT-qPCR (one-way ANOVA, P < 0.0001). f miR-4429 mimic/inhibitor or NC mimic/inhibitor transfected into DU145 and PC-3 cells for 48 h, and DLX1 protein expression was measured by western blot analysis (one-way ANOVA, P < 0. 0001) (see original western blots in Additional file 1: Figure S1). Data were expressed as mean ± SD from three independent experiments. *P < 0.05 was considered to show statistical significance